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Ladder three ladders Taken together, these outcomes recommend that LvRac1 is necessary in the innate immune response of shrimp to V. alginolyticus infection. Serine protease inhibitors, including Kunitz-kind protease inhibitors play important roles not solely in physiological process (i.e. blood clotting and fibrinolysis) but also in immune responses. In this study, we characterized a Kunitz-type protease inhibitor, designated MjKuPI, from kuruma shrimp Marsupenaeus japonicus. On this study, the cDNA of FREP was identified from the kuruma shrimp Marsupenaeus japonicus (MjFREP2). On this study, a cDNA encoding a RAS GTPase (LvRac1) in the Pacific white shrimp (L. Injection of the bacterium V. alginolyticus into L. vannamei induced hepatopancreatic upregulation of LvRac1 expression. Suppressing LvRac1 also promoted the upregulation of Lvp53, LvCu/ZnSOD, and Lvgal following V. alginolyticus injection. The silencing of LvRac1 by double-stranded RNA injection thus elevated the V. alginolyticus challenge sensitivity of L. vannamei and weakened its bacterial clearance ability in vivo. Moreover, knocking down LvRac1 in vivo considerably decreased the expression of the L. vannamei p53 and Cu/Zn superoxide dismutase genes (Lvp53 and LvCu/Zn SOD, respectively) whereas rising that of the galectin gene (Lvgal). What are good inquiries to ask a guy whereas enjoying the question game?